Difference between revisions of "Part:BBa K5175034:Design"
Andre-zeng (Talk | contribs) (Created page with " __NOTOC__ <partinfo>BBa_K5175035 short</partinfo> <partinfo>BBa_K5175035 SequenceAndFeatures</partinfo> ===Design Notes=== Nothing. ===Source=== <i>E.coli, Ideonella s...") |
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Revision as of 19:53, 1 October 2024
T7 promoter-lac operator-pelB-MHETase -G4S-FAST-PETase -T7 terminator-T7 promoter-fucO-aldA-T7 termi
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 826
Illegal PstI site found at 1169
Illegal PstI site found at 3117
Illegal PstI site found at 4865
Illegal PstI site found at 5578 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2820
Illegal NheI site found at 2876
Illegal NheI site found at 5636
Illegal PstI site found at 826
Illegal PstI site found at 1169
Illegal PstI site found at 3117
Illegal PstI site found at 4865
Illegal PstI site found at 5578 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 709
Illegal BamHI site found at 4403
Illegal XhoI site found at 1967 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 826
Illegal PstI site found at 1169
Illegal PstI site found at 3117
Illegal PstI site found at 4865
Illegal PstI site found at 5578 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 826
Illegal PstI site found at 1169
Illegal PstI site found at 3117
Illegal PstI site found at 4865
Illegal PstI site found at 5578
Illegal NgoMIV site found at 166
Illegal NgoMIV site found at 508
Illegal NgoMIV site found at 896
Illegal NgoMIV site found at 1259
Illegal AgeI site found at 3833
Illegal AgeI site found at 4034
Illegal AgeI site found at 4675 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Nothing.
Source
E.coli, Ideonella sakaiensis