Difference between revisions of "Part:BBa K5127000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli. | + | This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E. coli</i>. |
===Source=== | ===Source=== | ||
− | Genome of Vibrio fischeri. | + | Genome of <i>Vibrio fischeri</i>. |
Latest revision as of 10:03, 2 October 2024
VFA0359
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 250
Illegal SapI.rc site found at 544
Design Notes
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.
Source
Genome of Vibrio fischeri.
References
d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8
LaFleur, T. L., Hossain, A., & Salis, H. M. (2022). Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-32829-5
Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10(4), 354–360. https://doi.org/10.1038/nmeth.2404
Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9, 3617. https://doi.org/10.1038/s41467-018-05857-3
Taketani, M., Zhang, J., Zhang, S., Triassi, A. J., Huang, Y. J., Griffith, L. G., & Voigt, C. A. (2020). Genetic circuit design automation for the gut resident species Bacteroides thetaiotaomicron. Nature Biotechnology, 38(9), 962–969. https://doi.org/10.1038/s41587-020-0468-5