Difference between revisions of "Part:BBa K197015:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
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<partinfo>BBa_K197015 short</partinfo>
 
<partinfo>BBa_K197015 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===

Latest revision as of 05:19, 22 October 2009

{eCPX (circularly permuted OmpX)}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 189
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

 
PCR ca998/O09ig282 on M10014 	(477, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig282   	{<eCPX>}
-------------------------------------
Ca998  	forward oligo 
gtatcacgaggcagaatttcag
O09ig282 	reverse oligo with stop codons removed for {<eCPX>}
ccggtGGATCCAGAGCTTGCAGTACGGCTTT

References