Difference between revisions of "Part:BBa K257013:Experience"
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x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose | x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose | ||
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[[Image:PbadLacIYFP fluo.jpg|360px]] | [[Image:PbadLacIYFP fluo.jpg|360px]] | ||
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x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose | x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose | ||
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[[Image:PbadLacIYFP glucose trans1.jpg|360px]] | [[Image:PbadLacIYFP glucose trans1.jpg|360px]] | ||
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x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose | x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose | ||
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[[Image:PbadLacIYFP glucose fluo1.jpg|360px]] | [[Image:PbadLacIYFP glucose fluo1.jpg|360px]] | ||
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'''Conclusion :''' | '''Conclusion :''' | ||
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| + | In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly to the construction less bacteria with our construction are fluorescent in presence of glucose than in the control. | ||
Revision as of 03:58, 22 October 2009
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Applications of BBa_K257013
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose
Conclusion :
In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly to the construction less bacteria with our construction are fluorescent in presence of glucose than in the control.