Difference between revisions of "Part:BBa K5127002:Design"

 
Line 12: Line 12:
  
 
===Source===
 
===Source===
 +
Genome of Vibrio cholerae.
 +
  
−
Genome of Vibrio cholerae
 
  
 
===References===
 
===References===
 +
Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w
 +
 +
Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006

Revision as of 19:06, 1 October 2024


BreR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

Genome of Vibrio cholerae.


References

Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w

Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006