Difference between revisions of "Part:BBa K5322037"
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| − | In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed | + | In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7 for subsequent experiments. |
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==Sequence and Features== | ==Sequence and Features== | ||
<partinfo>BBa_K5322037 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5322037 SequenceAndFeatures</partinfo> | ||
Latest revision as of 12:18, 2 October 2024
pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7
Usage and Biology
In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7 for subsequent experiments.
Figure 1-1 Plasmid pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 683
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 552
Illegal NheI site found at 702
Illegal NheI site found at 725
Illegal NheI site found at 1475
Illegal SpeI site found at 683 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 683
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 683
- 1000COMPATIBLE WITH RFC[1000]