Difference between revisions of "Part:BBa K5301014"
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===Characterization=== | ===Characterization=== | ||
| − | We performed an in vitro mixing and incubation of sGFP1-10 with sGFP11 to achieve their binding. The images captured under a fluorescence microscope are shown in the figure below. | + | We performed an in vitro mixing and incubation of sGFP1-10 with sGFP11 to achieve their binding(in the form of sGFP1-10/11 tether <partinfo>BBa_K5301017<partinfo><partinfo>BBa_K5301018<partinfo>). The images captured under a fluorescence microscope are shown in the figure below. |
Revision as of 15:14, 1 October 2024
sGFP11 is an engineered version of the final strand of the GFP beta-barrel.
sGFP11 is the smaller fragment of the Split-GFP system, encompassing the final β-strand domain of green fluorescent protein (GFP).The isolated GFP11 fragment does not fluoresce on its own and is typically fused to the C-terminus of the target protein. Upon binding with the GFP1-10 fragment(BBa_K5301012), it facilitates the restoration of GFP's fluorescent activity.
Usage and Biology
In our team's experiments, sGFP1-10 and sGFP11 are individually fused and expressed with the target proteins.We use the self-assembly of GFP1-10 and GFP11 to construct membrane protein dimers.
Characterization
We performed an in vitro mixing and incubation of sGFP1-10 with sGFP11 to achieve their binding(in the form of sGFP1-10/11 tether No part name specified with partinfo tag.