Difference between revisions of "Part:BBa K197027:Design"
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===Source=== | ===Source=== | ||
| − | + | <pre> | |
| + | PCR Odvs0007F/ca1383 on CFT073 gen. (304bp, gp = A) | ||
| + | PCR ca1382/Odvs0008rR on CFT073 gen. (96bp, gp = B) | ||
| + | PCR Odvs0008rF/Odvs0007R on CFT073 gen. (89 bp, gp = C) | ||
| + | --------------------------------------------------- | ||
| + | PCR Odvs0007F/Odvs0007R on A+B+C (436bp, EcoRI/BamHI) | ||
| + | sub into pBca9495????? ..... | ||
| + | --------------------------------------------------- | ||
| + | Odvs0007F Forward EcoRI/BglII for Bglbrick of ECOHLY ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc | ||
| + | Odvs0007R Reverse BamHI for Bglbrick of ECOHLY ctgatGGATCCataaatatcattaccatatccacc | ||
| + | Odvs0008rF Removing the BglII site in ECOHLY (Forward) ggcagtgagggagcagaCctgcttgatggcgg | ||
| + | Odvs0008rR Removing the BglII site in ECOHLY (Reverse) ccgccatcaagcagGtctgctccctcactgcc | ||
| + | ca1382 Forward oligo to remove ecoRI site in beta roll GTTCAGGGcAATTCTCTTGC | ||
| + | ca1383 Reverse oligo to remove ecoRI site in beta roll GCAAGAGAATTgCCCTGAAC | ||
| + | </pre> | ||
===References=== | ===References=== | ||
Baumann U, Wu S, Flaherty KM, McKay DB. Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif. EMBO J. 1993 Sep;12(9):3357-64. PMID: 8253063 | Baumann U, Wu S, Flaherty KM, McKay DB. Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif. EMBO J. 1993 Sep;12(9):3357-64. PMID: 8253063 | ||
Revision as of 04:16, 22 October 2009
Beta Roll
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a beta roll
Source
PCR Odvs0007F/ca1383 on CFT073 gen. (304bp, gp = A) PCR ca1382/Odvs0008rR on CFT073 gen. (96bp, gp = B) PCR Odvs0008rF/Odvs0007R on CFT073 gen. (89 bp, gp = C) --------------------------------------------------- PCR Odvs0007F/Odvs0007R on A+B+C (436bp, EcoRI/BamHI) sub into pBca9495????? ..... --------------------------------------------------- Odvs0007F Forward EcoRI/BglII for Bglbrick of ECOHLY ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc Odvs0007R Reverse BamHI for Bglbrick of ECOHLY ctgatGGATCCataaatatcattaccatatccacc Odvs0008rF Removing the BglII site in ECOHLY (Forward) ggcagtgagggagcagaCctgcttgatggcgg Odvs0008rR Removing the BglII site in ECOHLY (Reverse) ccgccatcaagcagGtctgctccctcactgcc ca1382 Forward oligo to remove ecoRI site in beta roll GTTCAGGGcAATTCTCTTGC ca1383 Reverse oligo to remove ecoRI site in beta roll GCAAGAGAATTgCCCTGAAC
References
Baumann U, Wu S, Flaherty KM, McKay DB. Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif. EMBO J. 1993 Sep;12(9):3357-64. PMID: 8253063