Difference between revisions of "Part:BBa K243035:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | [https://static.igem.org/mediawiki/parts/d/de/Freiburg09_419.txt Commented GenBank file] | + | [https://static.igem.org/mediawiki/parts/d/de/Freiburg09_419.txt Commented GenBank file]<br> |
This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector. It has a weak RBS for a low expression level,an ampicilin resistance and lacI operon. | This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector. It has a weak RBS for a low expression level,an ampicilin resistance and lacI operon. | ||
Latest revision as of 03:25, 22 October 2009
pJS419
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 2266
Illegal XbaI site found at 1382 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2267
Illegal PstI site found at 2281
Illegal NotI site found at 2274 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1494
Illegal BamHI site found at 2365 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2267
Illegal XbaI site found at 1382 - 25INCOMPATIBLE WITH RFC[25]Illegal suffix found in sequence at 2257
Illegal XbaI site found at 1382
Illegal NgoMIV site found at 1390
Illegal NgoMIV site found at 2498
Illegal AgeI site found at 3832 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2114
Design Notes
Commented GenBank file
This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector. It has a weak RBS for a low expression level,an ampicilin resistance and lacI operon.