Difference between revisions of "Part:BBa K5492010"

Latest revision as of 14:58, 1 October 2024

Twist adapter forward primer

Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.

We have found the following PCR protocol the most optimal for the part:

We added the following substrates in order:

We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DAO solution in the following order:

1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder

Assembly Compatibility:

Revision as of 14:49, 1 October 2024 (view source) Noriii (Talk \| contribs) ← Older edit		Latest revision as of 14:58, 1 October 2024 (view source) Noriii (Talk \| contribs)
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	https://static.igem.wiki/teams/5492/registry/dao-pcr-substr.png		https://static.igem.wiki/teams/5492/registry/dao-pcr-substr.png
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