Difference between revisions of "Part:BBa K5492010"
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| − | + | ===Experiments=== | |
==PCR== | ==PCR== | ||
We have found the following PCR protocol the most optimal for the part: | We have found the following PCR protocol the most optimal for the part: | ||
Revision as of 14:28, 1 October 2024
Twist adapter forward primer
Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.
Experiments
PCR
We have found the following PCR protocol the most optimal for the part:
Optimised dao:
We added the following substrates in order:
We added the 6X DNA Loading dye to each PCR tubes.
We loaded the wells of the gels with 12μL of DAO solution in the following order:
1. D31O
2. D32O
3. D33O
4. D34O
5. Ladder
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]