Difference between revisions of "Part:BBa K197026:Design"

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===Source===
 
===Source===
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Construction of Ice Nucleation Repeats (INP repeats) Basic Part
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synthesis from Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340
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PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI)
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Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L)
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Product is pBca9495AK-M10048      {<INP repeats>}
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---
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eh001F Forward EcoRI oligo for <INP repeats>
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gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
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eh001R Reverse BamHI oligo for <INP repeats>
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gtcagGGATCCactgtcctgcccggccgttg
  
 
===References===
 
===References===
 
Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340
 
Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340

Revision as of 04:12, 22 October 2009

INP-Repeats


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a part of a repeat in the ice nucleation protien


Source

Construction of Ice Nucleation Repeats (INP repeats) Basic Part PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L) Product is pBca9495AK-M10048 {<INP repeats>} --- eh001F Forward EcoRI oligo for <INP repeats> gtcagGAATTCatgAGATCTaaccacagtgatctaattgc eh001R Reverse BamHI oligo for <INP repeats> gtcagGGATCCactgtcctgcccggccgttg

References

Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340