Difference between revisions of "Part:BBa K5115071"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We delete the stop codon of this part to link it with [https://parts.igem.org/Part:BBa_K5115085 BBa_K5115085(F1v)].For more information about their experiment, please check [https://parts.igem.org/Part:BBa_K5115086 BBa_K5115086(NixA-F1v | + | We delete the stop codon of this part to link it with [https://parts.igem.org/Part:BBa_K5115085 BBa_K5115085(F1v)].For more information about their experiment, please check [https://parts.igem.org/Part:BBa_K5115086 BBa_K5115086(NixA-F1v)]. |
===Characterization=== | ===Characterization=== | ||
Revision as of 12:54, 1 October 2024
NixA, without stop codon
Introduction
NixA is a high-affinity nickel transporter in helicobacter pylori. It belongs to the NiCoT family of transporters and facilitates the import of Ni²⁺ ions across the bacterial cytoplasmic membrane. These nickel ions make up the active sites of urease and hydrogenase, enzymes crucial for H. pylori's ability to survive in the highly acidic environment of the stomach.[1] In our design, the NixA is expressed on the cell membrane of E.coli and undertake the task of importing nickel into the bacteria.
Usage and Biology
We delete the stop codon of this part to link it with BBa_K5115085(F1v).For more information about their experiment, please check BBa_K5115086(NixA-F1v).
Characterization
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| Figure 1. The alphafold structure of NixA.[2]
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Fischer, F., Robbe-Saule, M., Turlin, E., Mancuso, F., Michel, V., Richaud, P., Veyrier, F. J., Reuse, H. D., & Vinella, D. (2016). Characterization in Helicobacter pylori of a Nickel Transporter Essential for Colonization That Was Acquired during Evolution by Gastric Helicobacter Species. PLOS Pathogens, 12(12), e1006018.
- ↑ Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C.-C., O’Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., … Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(8016), 493–500.