Difference between revisions of "Part:BBa K5322013"

Revision as of 12:42, 1 October 2024

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.

Usage and Biology

Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7,the pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.

Structural design

Our team designed the gene circuit shown in the figure below: pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.

Figure 1-1 gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Construction of the plasmid

Our team designed the plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7, and the plasmid map is illustrated below.

Figure 2-1 Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 552
Illegal NheI site found at 1157
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7
+Our team designed the plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7, and the plasmid map is illustrated below.
-The pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.
 </p>
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 <div class="center-img">
-<img src="https://static.igem.wiki/teams/5322/wet-lab/20-pet29a-j23119-rbs-mfp3-t7.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300">
+<img src="https://static.igem.wiki/teams/5322/t-imgs/1-pet29a-j23119-rbs-sod-1-t7.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300">
 <p align="center"><b>Figure 2-1</b> Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
 </div>