Difference between revisions of "Part:BBa K5291003"
| Line 5: | Line 5: | ||
<i>vgb</i> encodes VHb. VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to improve the degradation ability of CYPY96F on PE. | <i>vgb</i> encodes VHb. VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to improve the degradation ability of CYPY96F on PE. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:43, 1 October 2024
vgb: Vitreoscilla hemoglobin
vgb encodes VHb. VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to improve the degradation ability of CYPY96F on PE.
Usage and Biology
In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.
Fig.1 The map of plasmid pAB1-CYPY96F-VHB.
Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.
Fig.2 The SDS-PAGE result of CYPY96F.
Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]