Difference between revisions of "Part:BBa K194002"
 
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This variant of biobrick BBa_K194001 and improvement of <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040" target="_blank">BBa_E0040</a></html> has been destabilized by the fusion of biobrick BBa_K194000 and BBa_K194001.  
 
This variant of biobrick BBa_K194001 and improvement of <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040" target="_blank">BBa_E0040</a></html> has been destabilized by the fusion of biobrick BBa_K194000 and BBa_K194001.  
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In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.  
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In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes. <br><br>
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<b>References</b>
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Mateus and Avery, 2000. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast 2000; 16: 1313±1323.  
  
 
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Latest revision as of 02:54, 22 October 2009

Destabilized GFP for yeast

This variant of biobrick BBa_K194001 and improvement of BBa_E0040 has been destabilized by the fusion of biobrick BBa_K194000 and BBa_K194001. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.

References Mateus and Avery, 2000. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast 2000; 16: 1313±1323.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644