Difference between revisions of "Part:BBa K5302016"
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<partinfo>BBa_K5302002 short</partinfo>
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This work is derived from pBBR-OmpA-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pBBR-OmpA-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5302002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K5302002 parameters</partinfo>
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Revision as of 14:02, 1 October 2024


8IJZ

This work is derived from pBBR-OmpA-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. Colony PCR results of pBBR-OmpA-VEGFR1D2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]