Difference between revisions of "Part:BBa K5236016"

Latest revision as of 12:16, 2 October 2024

pET28a-pelB-IsPETase(H104W)-6xHis

This composite part codes for the mutated IsPETase H104W and expresses the enzyme in the extracellular space of chassis.

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

IsPETase H104W: This sequence encodes for a mutated IsPETase H104W and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription. "

Fig.1 The result of pET28a-pelB-IsPETase(H104W)-6xHis DNA sequencing

Fig.2 Constructed Plasmid

Sequence and Features

Assembly Compatibility:

@@ Line 21: / Line 21: @@
 <center><html><img src ="https://static.igem.wiki/teams/5236/part-images/h104w-sequence-cycle3.png" width = "50%"><br></html></center>
 <center>Fig.1 The result of pET28a-pelB-IsPETase(H104W)-6xHis  DNA sequencing  </center>
+<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/ispetase-h104w.png" width = "50%"><br></html></center>
+<center>Fig.2 Constructed Plasmid  </center>
 <!-- Add more about the biology of this part here