Difference between revisions of "Part:BBa K218018:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The plasmid containing | + | The plasmid containing LuxO D47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999). |
>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E). | >gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E). |
Revision as of 03:19, 22 October 2009
LuxO D47E with strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 136
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 824
Illegal SapI.rc site found at 786
Design Notes
The plasmid containing LuxO D47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999).
>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E).
Source
Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.