Difference between revisions of "Part:BBa K5291025"
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<i>pntA</i> encodes PntA, the &#945; subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogenase. It is composed of two domains, i.e. hydrophilic domain I containing the NAD(H) binding site and hydrophobic domain II containing the membrane spanning &#945;-helices. PntA can bind to membranes and plays a key role in stabilizing the overall conformation of the cytoplasmic moiety of pyridine nucleotide transferase.
 
<i>pntA</i> encodes PntA, the &#945; subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogenase. It is composed of two domains, i.e. hydrophilic domain I containing the NAD(H) binding site and hydrophobic domain II containing the membrane spanning &#945;-helices. PntA can bind to membranes and plays a key role in stabilizing the overall conformation of the cytoplasmic moiety of pyridine nucleotide transferase.
  
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===Usage and Biology===
 
===Usage and Biology===
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In carbon fixation module we constructed pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i> to improve the intracellular NADPH and NADP+ level.<br>
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<html><img width = "700" src="https://static.igem.wiki/teams/5291/images/parts-lhs/pbbr1mcs2-pnta-pntb-nadm-nadk.png" /></html><br>
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<b>Fig.1 The map of plasmid pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i>.</b><br><br>
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We successfully cloned the pntA-pntB gene by PCR.<br>
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<html><img width = "400" src="https://static.igem.wiki/teams/5291/images/result/electropherogram-of-nadm-nadk-pcr-gel.png" /></html><html><img width = "500" src="https://static.igem.wiki/teams/5291/images/parts-lhs/nadm-nadk-pnta-pntb.png" /></html><br>
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<b>Fig.2 Electropherogram of nadM-nadK PCR gel.</b>
  
 
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Revision as of 13:35, 1 October 2024


pntA: α subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogen

pntA encodes PntA, the α subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogenase. It is composed of two domains, i.e. hydrophilic domain I containing the NAD(H) binding site and hydrophobic domain II containing the membrane spanning α-helices. PntA can bind to membranes and plays a key role in stabilizing the overall conformation of the cytoplasmic moiety of pyridine nucleotide transferase.

Usage and Biology

In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP+ level.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

We successfully cloned the pntA-pntB gene by PCR.

Fig.2 Electropherogram of nadM-nadK PCR gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]