Difference between revisions of "Part:BBa K5291003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.<br> | ||
| + | <html><img width = "700" src="https://static.igem.wiki/teams/5291/images/part-wyn/cyp-plasmid.png" /></html><br> | ||
| + | <b>Fig.1 The map of plasmid pAB1-CYPY96F-VHB.</b><br><br> | ||
| + | Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.<br> | ||
| + | <html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-wyn/sds-page-cyp.png" /></html><br> | ||
| + | <b>Fig.2 The SDS-PAGE result of CYPY96F.</b><br><br> | ||
| + | Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.<br> | ||
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Revision as of 11:26, 1 October 2024
vgb: Vitreoscilla hemoglobin
vgb encodes VHb. VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to improve the degradation ability of CYPY96F on PE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]