Difference between revisions of "Part:BBa K5276010"
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MutaT7, initially a fusion of cytosine deaminase (AID) and T7 RNA polymerase (T7RNAP), enables mutations from dC→dT and deoxyguanosine (dG) to deoxyadenosine (dA) (caused by dC→dT mutations on the complementary strand). On this basis, when combined with adenosine deaminase, A→G and T→C mutations can be achieved (A:T→G:C). The enzyme, enhanced for mutagenic activity, is called eMutaT7(Park et al., 2021). | MutaT7, initially a fusion of cytosine deaminase (AID) and T7 RNA polymerase (T7RNAP), enables mutations from dC→dT and deoxyguanosine (dG) to deoxyadenosine (dA) (caused by dC→dT mutations on the complementary strand). On this basis, when combined with adenosine deaminase, A→G and T→C mutations can be achieved (A:T→G:C). The enzyme, enhanced for mutagenic activity, is called eMutaT7(Park et al., 2021). | ||
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Latest revision as of 08:05, 1 October 2024
eMutaT7
MutaT7, initially a fusion of cytosine deaminase (AID) and T7 RNA polymerase (T7RNAP), enables mutations from dC→dT and deoxyguanosine (dG) to deoxyadenosine (dA) (caused by dC→dT mutations on the complementary strand). On this basis, when combined with adenosine deaminase, A→G and T→C mutations can be achieved (A:T→G:C). The enzyme, enhanced for mutagenic activity, is called eMutaT7(Park et al., 2021).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]