Difference between revisions of "Part:BBa K5233044:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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TPA was not transported in wild C.elegans. so we transformed plasmids carrying tpiA and tpiB to transport TPA.
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the whole composite part is the transport system that could transport TPA into the engineering cell. Be aware that the composite part is designed for C. elegans, and may not function in prokaryotic system.
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===Source===
 
===Source===

Latest revision as of 03:51, 2 October 2024


vha6-gbb1-tpiA-SL2-glr2-tpiB-SL2-dsl1-tphC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2774
    Illegal PstI site found at 776
    Illegal PstI site found at 1405
    Illegal PstI site found at 3281
    Illegal PstI site found at 4459
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2774
    Illegal PstI site found at 776
    Illegal PstI site found at 1405
    Illegal PstI site found at 3281
    Illegal PstI site found at 4459
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3888
    Illegal BamHI site found at 2487
    Illegal BamHI site found at 3320
    Illegal BamHI site found at 4627
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2774
    Illegal PstI site found at 776
    Illegal PstI site found at 1405
    Illegal PstI site found at 3281
    Illegal PstI site found at 4459
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2774
    Illegal PstI site found at 776
    Illegal PstI site found at 1405
    Illegal PstI site found at 3281
    Illegal PstI site found at 4459
    Illegal NgoMIV site found at 2441
    Illegal NgoMIV site found at 4386
    Illegal AgeI site found at 1686
    Illegal AgeI site found at 2738
    Illegal AgeI site found at 2927
    Illegal AgeI site found at 3571
    Illegal AgeI site found at 3721
    Illegal AgeI site found at 4180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2600
    Illegal BsaI site found at 3433
    Illegal BsaI.rc site found at 303


Design Notes

the whole composite part is the transport system that could transport TPA into the engineering cell. Be aware that the composite part is designed for C. elegans, and may not function in prokaryotic system.

Source

The tpiA, tpiB and tphC come from Comamonas thiooxidans strain S23. The signal peptides come from Caenorhabditis elegans (strain: Bristol N2)

References