Difference between revisions of "Part:BBa K5302034"

 
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This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#29, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#29, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-o-vegfr1d2-l1masking29-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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            <b>Figure 1. </b> Colony PCR results of pBBR-OmpA-VEGFR1D2-l1masking#29
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:29, 1 October 2024


OmpA-VEGFR1D2-l1masking#29

This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#29, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. Colony PCR results of pBBR-OmpA-VEGFR1D2-l1masking#29

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2367
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2367
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NotI site found at 5299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2367
    Illegal BglII site found at 6045
    Illegal BamHI site found at 1302
    Illegal XhoI site found at 2308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2367
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2367
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 3025
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 2865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5621
    Illegal SapI.rc site found at 423
    Illegal SapI.rc site found at 633