Difference between revisions of "Part:BBa K5302024"

 
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This work is derived from pBBR1MCS-INP-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines INP(about 30kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pBBR1MCS-INP-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines INP(about 30kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-inp-vegfr-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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        <caption>
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            <b>Figure 1. </b> Colony PCR results of pBBR-INP-VEGFR1D2 （1）
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-inp-vegfr-2.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 2. </b> Colony PCR results of pBBR-INP-VEGFR1D2 （2）
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-p-inp-vegfr-3.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 3. </b> Colony PCR results of pBBR-INP-VEGFR1D2 （3）
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        </caption>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:14, 1 October 2024


pBBR-INP-VEGFR1D2

This work is derived from pBBR1MCS-INP-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines INP(about 30kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. Colony PCR results of pBBR-INP-VEGFR1D2 （1）

Jamboree Program
Figure 2. Colony PCR results of pBBR-INP-VEGFR1D2 （2）

Jamboree Program
Figure 3. Colony PCR results of pBBR-INP-VEGFR1D2 （3）

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4584
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4584
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4584
    Illegal BglII site found at 1803
    Illegal BamHI site found at 3195
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4584
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4584
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 3616
    Illegal NgoMIV site found at 5249
    Illegal AgeI site found at 4193
    Illegal AgeI site found at 5089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526