Difference between revisions of "Part:BBa K5322006:Design"
(→Source) |
(→References) |
||
| Line 17: | Line 17: | ||
===References=== | ===References=== | ||
| + | 1. Aich P, An J, Yang B, Ko Y H, Kim J, Murray J, Cha H J, Roh J H, Park K M, Kim K. Chem. Commun., 2018, 54(89): 12642. | ||
| + | |||
| + | 2. Ahn J M, Lee J S, Um S G, et al. Mussel adhesive protein-conjugated vitronectin (fp-151-VT) induces anti-inflamma-tory activity on LPS-stimulated macrophages and UVB-irra-diated keratinocytes[J]. Immunological Investigations. 2019,48: 242-254. | ||
Latest revision as of 13:20, 1 October 2024
NO-inducible Mfp5 Expression System
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 552
Illegal NheI site found at 926 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 763
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To ensure that the animal-derived mussel foot protein Mfp5 can be successfully expressed in Escherichia coli Nissle 1917, we performed codon optimization on the Mfp5 sequence.
Source
The plasmid pET29a-J23119-SoxR-T-pSoxS-RBS-Mfp5-T7 utilizes the pET29a vector, which is commonly used for high-level gene expression in Escherichia coli. The components used in this design, including the J23119 promoter, Mfp5, SoxR protein, and SoxS promoter, are derived from standard biological parts registered in the iGEM registry (BBa_J23119, BBa_K1583002, BBa_K554003 , BBa_K5322004), with the mussel foot protein Mfp5 sourced from natural marine mussels. These parts were selected due to their validated functions and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions.
References
1. Aich P, An J, Yang B, Ko Y H, Kim J, Murray J, Cha H J, Roh J H, Park K M, Kim K. Chem. Commun., 2018, 54(89): 12642.
2. Ahn J M, Lee J S, Um S G, et al. Mussel adhesive protein-conjugated vitronectin (fp-151-VT) induces anti-inflamma-tory activity on LPS-stimulated macrophages and UVB-irra-diated keratinocytes[J]. Immunological Investigations. 2019,48: 242-254.