Difference between revisions of "Part:BBa K5302016"
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+ | This work is derived from pBBR-OmpA-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence. | ||
+ | <html> | ||
+ | <div style="text-align:center;"> | ||
+ | <img src="https://static.igem.wiki/teams/5302/images/part-registry-povegfr1d2-1.png" | ||
+ | width="60%" style="display:block; margin:auto;" alt="Jamboree Program" > | ||
+ | <div style="text-align:center;"> | ||
+ | <caption> | ||
+ | <b>Figure 1. </b> Colony PCR results of pBBR-OmpA-VEGFR1D2 | ||
+ | </caption> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> |
Revision as of 10:59, 1 October 2024
This work is derived from pBBR-OmpA-mCherry and pUC19-VEGFR1D2, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.