Difference between revisions of "Part:BBa K239009:Experience"
(→Applications of BBa_K239009) |
(→User Reviews) |
||
| Line 20: | Line 20: | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K239009 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K239009 EndReviews</partinfo> | ||
| + | |||
| + | The device responded to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor. | ||
| + | |||
| + | Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase. | ||
| + | |||
| + | Detailed information please check [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II] | ||
Revision as of 01:52, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K239009
This part was designed to test promoter spy, BBa_K239001. It can be used to detect misfolding of proteins in the periplasm or shear stress in bio-processing.
User Reviews
UNIQfe4afc012fa156a8-partinfo-00000000-QINU UNIQfe4afc012fa156a8-partinfo-00000001-QINU
The device responded to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor.
Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase.
Detailed information please check [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II]