Difference between revisions of "Part:BBa K173010:Experience"
(→‎Materials)
 
Line 13: Line 13:
 
===='''Materials'''====
 
===='''Materials'''====
 
*Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
 
*Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
−
*Antibiotics were: Ampicillin (Amp) 100 ug/ml, Kanamycin (Kan) 50 ug/ml and Chloramphenicol (Cm) 12.5 ug/ml. All of them were stored at -20°C in 1000x stocks. Amp and Kan were dissolved in water, while Cm was dissolved in ethanol 100%.
+
*Antibiotics were: Ampicillin (Amp) 100 ug/ml. All of them were stored at -20°C in 1000x stocks.
 
*LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
 
*LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
−
*M9 supplemented medium was prepared according to: [http://openwetware.org/wiki/Knight:M9_supplemented_media Openwetware protocol].
 
−
*3OC6-HSL (Sigma) was dissolved in water and stored at -20°C in a 2mM stock.
 
−
*aTc (Clontech) was dissolved in ethanol 50% and stored at -20°C in a 100 ug/ml stock. All the following dilutions were performed in water.
 
 
*Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.
 
*Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.
−
 
−
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 13:53, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K173010

This part was not working in a preliminary induction test.



Materials

  • Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
  • Antibiotics were: Ampicillin (Amp) 100 ug/ml. All of them were stored at -20°C in 1000x stocks.
  • LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
  • Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.

User Reviews

UNIQb42e50587c26e409-partinfo-00000000-QINU UNIQb42e50587c26e409-partinfo-00000001-QINU