Difference between revisions of "Part:BBa K5301022"
Line 5: | Line 5: | ||
This pathway achieving self-cyclization of NW50 to construct nanodiscs with larger particle sizes. | This pathway achieving self-cyclization of NW50 to construct nanodiscs with larger particle sizes. | ||
+ | <center><html><img src='https://static.igem.wiki/teams/5301/parts/spytag-nw50-spycatcher.png' width='400px'></html></center> | ||
+ | <center><html>Figure 1.Structure of Spycatcher-NW50-SpyTag protein extract.From this structure diagram, it is predicted that spytag and spycatcher can be successfully combined at both ends of the NW50. | ||
+ | </html></center> | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 05:05, 1 October 2024
SpyCatcher-spNW50-SpyTag achieving self-cyclization of MSP nanodisc.
This pathway achieving self-cyclization of NW50 to construct nanodiscs with larger particle sizes.
Usage and Biology
The following figure shows the structure of the composite part(Figure 1).
<a href="" class="image"><img alt="" src="" width="100%" height=auto class="thumbimage" /></a>
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal NheI site found at 133
Illegal NheI site found at 2061
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal BglII site found at 993
Illegal BglII site found at 1533
Illegal BglII site found at 1701
Illegal BamHI site found at 166 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 565
Illegal EcoRI site found at 1645
Illegal SpeI site found at 517
Illegal PstI site found at 733
Illegal PstI site found at 1273
Illegal PstI site found at 1306
Illegal PstI site found at 1813 - 1000COMPATIBLE WITH RFC[1000]