Difference between revisions of "Part:BBa K5302011:Design"

 
 
Line 14: Line 14:
 
===Source===
 
===Source===
  
−
Source Organism:
+
mCherry:Mushroom coral
−
Escherichia coli BL21(DE3)
+
OmpA:Escherichia coli (strain K12)
−
equivalent:
+
−
Escherichia coli str. BL21(DE3)
+
  
 
===References===
 
===References===

Latest revision as of 13:30, 1 October 2024


pBBR-OmpA-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3884
    Illegal EcoRI site found at 3932
    Illegal EcoRI site found at 4205
    Illegal EcoRI site found at 4608
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3884
    Illegal EcoRI site found at 3932
    Illegal EcoRI site found at 4205
    Illegal EcoRI site found at 4608
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4193
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3884
    Illegal EcoRI site found at 3932
    Illegal EcoRI site found at 4205
    Illegal EcoRI site found at 4608
    Illegal BglII site found at 1803
    Illegal BamHI site found at 3195
    Illegal XhoI site found at 4550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3884
    Illegal EcoRI site found at 3932
    Illegal EcoRI site found at 4205
    Illegal EcoRI site found at 4608
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4193
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3884
    Illegal EcoRI site found at 3932
    Illegal EcoRI site found at 4205
    Illegal EcoRI site found at 4608
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4193
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 5266
    Illegal AgeI site found at 4411
    Illegal AgeI site found at 5106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.



Source

mCherry:Mushroom coral OmpA:Escherichia coli (strain K12)

References