Difference between revisions of "Part:BBa K5097011"

Revision as of 13:28, 2 October 2024

Acid-sensor, Base-producer pASR-PUT1

Usage and Biology

This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors.

Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 2.

This part contains the following: BBa K1231000 pASR

BBa_B0034

BBa_K5097009 SpeA

BBa_B0034

BBa_K5097010 SpeB

References

1-Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.

2-Charlier, Daniel & Bervoets, Indra. (2019). Regulation of arginine biosynthesis, catabolism and transport in Escherichia coli. Amino Acids. 51. 10.1007/s00726-019-02757-8.

Sequence and Features