Difference between revisions of "Part:BBa K5236014"
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<center>Fig.1 The result of DNA sequencing  </center>
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<center>Fig.1 The result of pET28a-pelB-IsPETase(M10L)-6xHis DNA sequencing  </center>
  
 
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Revision as of 09:01, 1 October 2024


pET28a-pelB-IsPETase(M10L)-6xHis

This composite part codes for the mutated IsPETase M10L and expresses the enzyme in the extracellular space of chassis.

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

IsPETase M10L: This sequence encodes for a mutated IsPETase M10L and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription.


Fig.1 The result of pET28a-pelB-IsPETase(M10L)-6xHis DNA sequencing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005