Difference between revisions of "Part:BBa K5236014"

Revision as of 02:49, 1 October 2024

pET28a-pelB-IsPETase(M10L)-6xHis

This composite part codes for the mutated IsPETase M10L and expresses the enzyme in the extracellular space of chassis.

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

IsPETase M10L: This sequence encodes for a mutated IsPETase M10L and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription.

Fig.1 The result of DNA sequencing

Sequence and Features

Assembly Compatibility:

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5236014 short</partinfo>
-This composite part codes for the mutated IsPETase M10L and allows the enzyme to exit the membrance of chassis.
+This composite part codes for the mutated IsPETase M10L and expresses the enzyme in the extracellular space of chassis.
 T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.