Difference between revisions of "Part:BBa K5236019"
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<partinfo>BBa_K5236019 short</partinfo> | <partinfo>BBa_K5236019 short</partinfo> | ||
| − | + | This composite part codes for the mutated BhrPETase W229F and expresses the enzyme in the extracellular space of chassis. | |
| + | |||
| + | T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins. | ||
| + | |||
Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG. | Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG. | ||
| + | |||
RBS: ribosome binding site. | RBS: ribosome binding site. | ||
| + | |||
BhrPETase W229F: This sequence encodes for a mutated BhrPETase W229F and is derived from Escherichia coli. | BhrPETase W229F: This sequence encodes for a mutated BhrPETase W229F and is derived from Escherichia coli. | ||
| + | |||
pelB: The sequence encodes a signal peptide that enables secretory expression of PETase. | pelB: The sequence encodes a signal peptide that enables secretory expression of PETase. | ||
| + | |||
6xHis: A label for protein purification | 6xHis: A label for protein purification | ||
| − | T7 terminator: Terminates transcription. | + | |
| + | T7 terminator: Terminates transcription. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 03:50, 1 October 2024
pET28a-pelB-BhrPETase(W229F)-6xHis
This composite part codes for the mutated BhrPETase W229F and expresses the enzyme in the extracellular space of chassis.
T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.
Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.
RBS: ribosome binding site.
BhrPETase W229F: This sequence encodes for a mutated BhrPETase W229F and is derived from Escherichia coli.
pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.
6xHis: A label for protein purification
T7 terminator: Terminates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 277
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 187
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 881