Difference between revisions of "Part:BBa K5236014"

Revision as of 02:46, 1 October 2024

pET28a-pelB-IsPETase(M10L)-6xHis

This composite part codes for the mutated IsPETase M10L and allows the enzyme to exit the membrance of chassis.

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

IsPETase M10L: This sequence encodes for a mutated IsPETase M10L and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription.

Fig.1 The result of DNA sequencing

Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K5236014 short</partinfo>
+This composite part codes for the mutated IsPETase M10L and allows the enzyme to exit the membrance of chassis.
 T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.
@@ Line 16: / Line 18: @@
 T7 terminator: Terminates transcription.
+<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/m10l-sequence-cycle-3.png" width = "50%"><br></html></center>
+<center>Fig.1 The result of DNA sequencing  </center>
 <!-- Add more about the biology of this part here