Difference between revisions of "Part:BBa K5236016"

Revision as of 03:23, 1 October 2024

pET28a-pelB-IsPETase(H104W)-6xHis

This composite part codes for the mutated IsPETase H104W and expresses the enzyme in the extracellular space of chassis.

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

IsPETase H104W: This sequence encodes for a mutated IsPETase H104W and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription. "

Fig.1 The result of DNA sequencing

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K5236016 short</partinfo>
+This composite part codes for the mutated IsPETase H104W and expresses the enzyme in the extracellular space of chassis.
 T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.
 Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.
 RBS: ribosome binding site.
 IsPETase H104W: This sequence encodes for a mutated IsPETase H104W and is derived from Escherichia coli.
 pelB:  The sequence encodes a signal peptide that enables secretory expression of PETase.
 xHis: A label for protein purification
 T7 terminator: Terminates transcription. "
+<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/h104w-sequence-cycle3.png" width = "50%"><br></html></center>
+<center>Fig.1 The result of DNA sequencing  </center>
 <!-- Add more about the biology of this part here