Difference between revisions of "Part:BBa K5115051"
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====Agarose gel electrophoresis====
 
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| '''Figure 1. Agarose gel electrophoresis of PCR products amplified from one ''E. coli'' (DH5α) colony.  
 
| '''Figure 1. Agarose gel electrophoresis of PCR products amplified from one ''E. coli'' (DH5α) colony.  

Revision as of 08:21, 1 October 2024


ribozyme connected hox and hyp

contributed by Fudan iGEM 2023

Introduction

This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. To learn more about the hydrogenase, please check BBa_K5115020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

Characterization

Agarose gel electrophoresis

Figure 1. Agarose gel electrophoresis of PCR products amplified from one E. coli (DH5α) colony.

M: DNA Marker. Lanes 1-8: Corresponding bands for hoxF, hoxU, hoxI, hoxH, hoxW, hoxY, hypA, and hypB, demonstrating successful assembly and integrity of the ribozyme-connected hox and hyp operon as designed. Primers for these PCR are listed on https://2024.igem.org/fudan/parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 654
    Illegal BglII site found at 1472
    Illegal BglII site found at 1765
    Illegal BglII site found at 2412
    Illegal BglII site found at 2490
    Illegal BamHI site found at 2883
    Illegal XhoI site found at 99
    Illegal XhoI site found at 2420
    Illegal XhoI site found at 2612
    Illegal XhoI site found at 3090
    Illegal XhoI site found at 5329
    Illegal XhoI site found at 7158
    Illegal XhoI site found at 8320
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1071
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1593
    Illegal NgoMIV site found at 3183
    Illegal NgoMIV site found at 3863
    Illegal NgoMIV site found at 6470
    Illegal AgeI site found at 2373
    Illegal AgeI site found at 7645
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2194
    Illegal BsaI site found at 2356
    Illegal BsaI site found at 4983
    Illegal BsaI.rc site found at 702
    Illegal BsaI.rc site found at 1206
    Illegal SapI.rc site found at 2305


References