Difference between revisions of "Part:BBa K5115051"
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| <html><img style="width:400px" src="https://static.igem.wiki/teams/5115/registry/ribozyme-connected-hox-and-hyp.png" alt="contributed by Fudan iGEM 2024"></html> | | <html><img style="width:400px" src="https://static.igem.wiki/teams/5115/registry/ribozyme-connected-hox-and-hyp.png" alt="contributed by Fudan iGEM 2024"></html> | ||
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| − | | '''"Figure 1. Agarose gel electrophoresis of PCR products amplified from one | + | | '''"Figure 1. Agarose gel electrophoresis of PCR products amplified from one ''E. coli'' (DH5α) colony. |
M: DNA Marker. Lanes 1-8: Corresponding bands for hoxF, hoxU, hoxI, hoxH, hoxW, hoxY, hypA, and hypB, demonstrating successful assembly and integrity of the ribozyme-connected hox and hyp operon as designed. Primers for these PCR are listed on https://2024.igem.org/fudan/parts." | M: DNA Marker. Lanes 1-8: Corresponding bands for hoxF, hoxU, hoxI, hoxH, hoxW, hoxY, hypA, and hypB, demonstrating successful assembly and integrity of the ribozyme-connected hox and hyp operon as designed. Primers for these PCR are listed on https://2024.igem.org/fudan/parts." | ||
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Revision as of 01:53, 1 October 2024
ribozyme connected hox and hyp
Introduction
This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. To learn more about the hydrogenase, please check BBa_K5115020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .
Characterization
Agarose gel electrophoresis
|
| "Figure 1. Agarose gel electrophoresis of PCR products amplified from one E. coli (DH5α) colony.
M: DNA Marker. Lanes 1-8: Corresponding bands for hoxF, hoxU, hoxI, hoxH, hoxW, hoxY, hypA, and hypB, demonstrating successful assembly and integrity of the ribozyme-connected hox and hyp operon as designed. Primers for these PCR are listed on https://2024.igem.org/fudan/parts." |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 654
Illegal BglII site found at 1472
Illegal BglII site found at 1765
Illegal BglII site found at 2412
Illegal BglII site found at 2490
Illegal BamHI site found at 2883
Illegal XhoI site found at 99
Illegal XhoI site found at 2420
Illegal XhoI site found at 2612
Illegal XhoI site found at 3090
Illegal XhoI site found at 5329
Illegal XhoI site found at 7158
Illegal XhoI site found at 8320 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 533
Illegal NgoMIV site found at 1071
Illegal NgoMIV site found at 1281
Illegal NgoMIV site found at 1593
Illegal NgoMIV site found at 3183
Illegal NgoMIV site found at 3863
Illegal NgoMIV site found at 6470
Illegal AgeI site found at 2373
Illegal AgeI site found at 7645 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2194
Illegal BsaI site found at 2356
Illegal BsaI site found at 4983
Illegal BsaI.rc site found at 702
Illegal BsaI.rc site found at 1206
Illegal SapI.rc site found at 2305