Difference between revisions of "Part:BBa K5322006:Design"

 
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===Design Notes===
 
===Design Notes===
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To ensure that the animal-derived mussel foot protein Mfp5 can be successfully expressed in <i>Escherichia coli</i> Nissle 1917, we performed codon optimization on the Mfp5 sequence.
  
  
  
 
===Source===
 
===Source===
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The plasmid pET29a-J23119-SoxR-T-pSoxS-RBS-Mfp5-T7 utilizes the pET29a vector, which is commonly used for high-level gene expression in <i>Escherichia coli</i>. The components used in this design, including the J23119 promoter, Mfp5, SoxR protein, and SoxS promoter, are derived from standard biological parts registered in the iGEM registry (<a href="https://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>, <a href="https://parts.igem.org/Part:BBa_K1583002">BBa_K1583002</a>, <a href="https://parts.igem.org/Part:BBa_K554003">BBa_K554003</a> , <a href="https://parts.igem.org/Part:BBa_K5322004">BBa_K5322004</a>), with the mussel foot protein Mfp5 sourced from natural marine mussels. These parts were selected due to their validated functions and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions.
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===References===
 
===References===

Revision as of 04:35, 1 October 2024


NO-inducible Mfp5 Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 552
    Illegal NheI site found at 926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure that the animal-derived mussel foot protein Mfp5 can be successfully expressed in Escherichia coli Nissle 1917, we performed codon optimization on the Mfp5 sequence.


Source

The plasmid pET29a-J23119-SoxR-T-pSoxS-RBS-Mfp5-T7 utilizes the pET29a vector, which is commonly used for high-level gene expression in Escherichia coli. The components used in this design, including the J23119 promoter, Mfp5, SoxR protein, and SoxS promoter, are derived from standard biological parts registered in the iGEM registry (BBa_J23119, BBa_K1583002, BBa_K554003 , BBa_K5322004), with the mussel foot protein Mfp5 sourced from natural marine mussels. These parts were selected due to their validated functions and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions.

References