Difference between revisions of "Part:BBa K5322031"

 
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__TOC__
  
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===Usage and Biology===
 
  
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==Usage and Biology==
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5322031 SequenceAndFeatures</partinfo>
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The complete mouse PD-L1 protein has a large molecular weight and complex structure, making it challenging to express in the prokaryotic organism <i>Escherichia coli</i>. Therefore, we opted for a truncated version of the PD-L1 functional domain, designing an expression system for the PD-L1 functional domain: pET29a-J23119-RBS-PD-L1 (functional domain)-T7. The plasmid diagram is shown below.
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<img src="https://static.igem.wiki/teams/5322/wet-lab/pet29a-j23119-rbs-pd-l1-functional-domain-t7-map.png" alt="pET29a-J23119-RBS-PD-L1 Functional domain -T7" width="300">
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<p align="center"><b>Figure 1-1</b>  Plasmid pET29a-J23119-RBS-PD-L1 Functional domain -T7</p>
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==Construction of the plasmid==
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===Functional Parameters===
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==Sequence and Features==
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<partinfo>BBa_K5322031 SequenceAndFeatures</partinfo>

Revision as of 13:10, 1 October 2024


Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System


Usage and Biology

The complete mouse PD-L1 protein has a large molecular weight and complex structure, making it challenging to express in the prokaryotic organism Escherichia coli. Therefore, we opted for a truncated version of the PD-L1 functional domain, designing an expression system for the PD-L1 functional domain: pET29a-J23119-RBS-PD-L1 (functional domain)-T7. The plasmid diagram is shown below.

pET29a-J23119-RBS-PD-L1 Functional domain -T7

Figure 1-1 Plasmid pET29a-J23119-RBS-PD-L1 Functional domain -T7

Construction of the plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 519
    Illegal PstI site found at 377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]