Difference between revisions of "Part:BBa K5301021"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We used this pathway to express SnTST- mCh[11] by using T7 expression system in <i>E.coli</i>. And the expression of SnTST- mCh[11] can be induced by IPTG. For more information on SnTST- mCh[11], go to <partinfo> | + | We used this pathway to express SnTST- mCh[11] by using T7 expression system in <i>E.coli</i>. And the expression of SnTST- mCh[11] can be induced by IPTG. For more information on SnTST- mCh[11], go to <partinfo>BBa_K5301007</partinfo>. |
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Revision as of 00:32, 1 October 2024
SnTST-mCh[11] expression pathway (T7)
This pathway expresses SnTST- mCh[11] using T7 expression system.
Usage and Biology
We used this pathway to express SnTST- mCh[11] by using T7 expression system in E.coli. And the expression of SnTST- mCh[11] can be induced by IPTG. For more information on SnTST- mCh[11], go to BBa_K5301007.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1002
Illegal NotI site found at 815 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 334
Illegal BglII site found at 706 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]