Difference between revisions of "Part:BBa K5439006"
Line 9: Line 9:
 
<div style="text-align:justify;">
 
<div style="text-align:justify;">
  
−
To create an biosensor capable of detecting  
+
To create an biosensor capable of detecting the anti-inflammatory drug ibuprofen, the FRET-based sensor system [https://parts.igem.org/Part:BBa_K1907000 (BBa_K4447004)] changing the middle part of the construct instead of the gene [https://parts.igem.org/Part:BBa_K1907000 (BBa_K4447001)], being the gene of interest [https://parts.igem.org/Part:BBa_K5439005 (BBa_K5439005)]; changing the
−
 
+
−
[1]. Gibson, D. G., Young, L., Chuang, R.-Y., Venter, J. C., Hutchison, C. A., & Smith, H. O. (2009). Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods, 6(5), 343–345. https://doi.org/10.1038/nmeth.1318
+

Revision as of 00:31, 1 October 2024


FRET-based system for the detection of ibuprofen

FRET-based sensor system for the detection of ibuprofen that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.(BBa_K5439005),an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP(BBa_K1159302)as energy donor and mVenus(BBa_K1907000)as an energy acceptor

Usage and Biology

To create an biosensor capable of detecting the anti-inflammatory drug ibuprofen, the FRET-based sensor system (BBa_K4447004) changing the middle part of the construct instead of the gene (BBa_K4447001), being the gene of interest (BBa_K5439005); changing the