Difference between revisions of "Part:BBa K5508006:Design"
 
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   <div class="unterschrift"><b> pESC-nueB-age</b>
 
   <div class="unterschrift"><b> pESC-nueB-age</b>
 
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   <div class="unterschrift"><b> ADH1-neuB-Gal1,10-age-CYC1</b>
 
   <div class="unterschrift"><b> ADH1-neuB-Gal1,10-age-CYC1</b>
 
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Latest revision as of 20:43, 30 September 2024


ADH1-neuB-Gal1,10-age-CYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 374
    Illegal AgeI site found at 1905
    Illegal AgeI site found at 2291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first verified the synthetic pathway from N-acetylglucosamine to N-acetylneuraminic acid, for which we needed to construct the plasmid pESC-neuB-age. The two genes were inserted in different directions of the bidirectional promoter in pESC-URA for simultaneous expression.

pESC-nueB-age

ADH1-neuB-Gal1,10-age-CYC1

Source

None

References