Difference between revisions of "PBAD Promoter Family"

Line 1: Line 1:
 +
__NOTOC__
 +
<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 680px; align: center">
 +
<center>
 +
[[pBAD Promoter Family |Family:]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
[[Part:BBa_K206000 |pBAD strong]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
[[Part:BBa_I13453 |pBAD wild type]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
[[Part:BBa_K206001 |pBAD weak]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</center>
 +
</div>
 
==Overview==
 
==Overview==
 
[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:
 
[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:
Line 10: Line 19:
 
<partinfo>K206001</partinfo>: pBAD weak
 
<partinfo>K206001</partinfo>: pBAD weak
  
 +
[[Image:PBAD family - Sequences.png|center|frame|Alignment of the mutated bases in pBAD weak and pBAD strong compared to I13453.]]
 
==Characterization==
 
==Characterization==
[[Image:PBAD family - Sequences.png|frame|Alignment of the mutated bases in pBAD weak and pBAD strong compared to I13453.]]
+
See the individual parts pages for details on characterization.
 +
===Transfer Function===
 +
===Expression Rate===
 +
===Specificity===
 +
[[Image:PBAD family - Specificity.png|center|frame|All of the promoters in the pBAD family respond specifically to L-arabinose.]]

Revision as of 00:59, 22 October 2009

Family:        pBAD strong        pBAD wild type        pBAD weak       

Overview

[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:

BBa_K206000: pBAD strong
BBa_I13453: pBAD wild type
BBa_K206001: pBAD weak
Alignment of the mutated bases in pBAD weak and pBAD strong compared to I13453.

Characterization

See the individual parts pages for details on characterization.

Transfer Function

Expression Rate

Specificity

All of the promoters in the pBAD family respond specifically to L-arabinose.