Difference between revisions of "Part:BBa K5150000"

Revision as of 08:25, 2 October 2024

(Bioactive multipeptide 3 (Optimized for E.coli)

This multiprotein peptide was designed to incorporate peptides with antidiabetic, antihypertensive, hypocholesterolemic, and antithrombotic properties. The goal was to achieve the simultaneous expression of multiple peptides by combining their sequences into a single gene. This strategy allows for the transcription and translation of a multiprotein peptide, with the individual peptides released through protease activity. It was optimized for high expression in E. coli.

The peptides exert their effects by modulating key metabolic pathways linked to diabetes and its complications. The protein can be used experimentally in projects involving nutrition studies, therapeutic natural products, food development, or the investigation of digestive proteases.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

**Figure 1. Multipeptide sequence 1 for *E.coli*.**

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5150000</partinfo>
-==Multipeptide sequence 3 (Optimized for ''E.coli'')==
+== (Bioactive multipeptide 3 (Optimized for ''E.coli'')==
+This multiprotein peptide was designed to incorporate peptides with antidiabetic, antihypertensive, hypocholesterolemic, and antithrombotic properties. The goal was to achieve the simultaneous expression of multiple peptides by combining their sequences into a single gene. This strategy allows for the transcription and translation of a multiprotein peptide, with the individual peptides released through protease activity. It was optimized for high expression in ''E. coli''.
+The peptides exert their effects by modulating key metabolic pathways linked to diabetes and its complications. The protein can be used experimentally in projects involving nutrition studies, therapeutic natural products, food development, or the investigation of digestive proteases.
-This multipeptide protein was carefully designed to integrate peptides with antidiabetic, antihypertensive, hypocholesterolemic and antithrombotic properties from food sources such as quinoa, soybean, goat milk, cow milk and calpis. It is optimized to be highly expressed in ''E. coli'' enabling straightforward purification through affinity chromatography.
-The main objective is to encapsulate the protein for oral ingestion so it could be released in the gut. Once released, the bioactive peptides are absorbed into the bloodstream, where they can modulate key metabolic pathways linked to diabetes and its complications. The protein can be used in projects related to nutrition studies, therapeutic natural products, food development or investigation of digestive proteases.