Difference between revisions of "Part:BBa K5097010"
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:::::::::Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019) | :::::::::Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019) | ||
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| + | ===References=== | ||
| + | Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0. | ||
Latest revision as of 18:14, 30 September 2024
SpeB (Agmatinase)
Usage and Biology
This part codes for the SpeB gene from E.coli. The gene codes for agmatine ureohydrolase. The enzyme is responsible for the catabolism of agmatine. It converts agmatine into urea and putrescine, and is the second step in the metabolic pathway that converts arginine into putrescine (Morris., et 1969). The 2024 Oneonta iGEM team designed the sequence for use in a base producing circuit. When co-expressed with SpeA (BBa_K5097010) it will construct the pathway from turning arginine into putrescine. Acidic conditions will be neutralized with the production of putrescine.
- Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)
References
Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 789
Illegal BamHI site found at 702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 724
- 1000COMPATIBLE WITH RFC[1000]
This gene sequence has been altered to make the SpeB gene RCF10 compatible. To do this, we removed an internal PstI restriction site, changing the nucleotide at position 318 from a G to an A, without affecting the amino acid sequence.