Difference between revisions of "Part:BBa K5136234"

 
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==<b>Biology</b>==
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===LexRO===
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LexRO is a synthetic light-switchable repressor, based on a novel LOV light sensor domain, RsLOV. In the darkness, LexRO dimerizes and binds to its cognate operator sequence to repress promoter activity. Upon light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates gene expression.
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===Usage and Biology===
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===SD7===
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A SD sequence upstream of LexRO encoding gene. SD7 is a ribosome binding site on the genome , which is capable of recruiting ribosomes in engineered <i>E. coli.</i>
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===<b>Characterization</b>===
  
 
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Revision as of 08:32, 2 October 2024


J23106-SD7-LexRO-B0015

Biology

LexRO

LexRO is a synthetic light-switchable repressor, based on a novel LOV light sensor domain, RsLOV. In the darkness, LexRO dimerizes and binds to its cognate operator sequence to repress promoter activity. Upon light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates gene expression.

SD7

A SD sequence upstream of LexRO encoding gene. SD7 is a ribosome binding site on the genome , which is capable of recruiting ribosomes in engineered E. coli.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]