Difference between revisions of "Part:BBa K5322011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence. | + | To ensure the successful expression of SOD-1 in <i>Escherichia coli</i>, we performed codon optimization of the SOD-1 sequence. |
===Source=== | ===Source=== | ||
| − | The team obtains the SOD-1 sequence from BBa_K2215003. | + | <html> |
| − | + | The team obtains the SOD-1 sequence from <a href="https://parts.igem.org/Part:BBa_K2215003">BBa_K2215003</a>. | |
| + | </html> | ||
===References=== | ===References=== | ||
Latest revision as of 12:33, 1 October 2024
pET-29(a)-pT7-lac operator-(SOD-1+His)-T7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 577
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
Source
The team obtains the SOD-1 sequence from BBa_K2215003.