Difference between revisions of "Part:BBa K5301023"

Revision as of 18:26, 30 September 2024

This pathway expresses SCSdC-mCh[1-10] using T7 expression system.

Usage and Biology

We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in E.coli. And the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to BBa_K5301005.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1764
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1283
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1079
Illegal AgeI site found at 1872
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in <i>E.coli</i>. And the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to <partinfo>BBa_K5301005</partinfo>.
-===Characterization===
-We used SDS-PAGE to test whether this pathway is expressed successfully(Figure 1). The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa. And we purified the target protein with a molecular weight of approximately 70-100 kD (Lane 4 - Lane 5), which demonstrated that we had successfully expressed this pathway.
-<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:min-content;"><div style="zoom:0.5;overflow:hidden;">
-https://static.igem.wiki/teams/5301/parts/sds-page-result-of-sdycatcher-for-sdycatcher.png
-</div><div class="thumbcaption">
-Figure 1.SDS-PAGE analysis of the express results of SCSdC-mCh[1-10] (containing SdyCatcher).The gel was run at 80 V for 10 minutes and then at 150 V for 20 minutes, followed by staining with Coomassie Brilliant Blue. The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa.
-</div></div></div></div>
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